Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides

Abstract Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.

Surgical research in the north and northeast of Brazil

PURPOSE: To evaluate the scientific production from the surgical areas from the State University of Para (UEPA), Federal Universities of Para (UFPA) and of Paraiba (UFPB), Brazil. METHODS: The scientific productions of professors in the last five years were classified according to the Qualis system of CAPES-Medicine III (http://qualis.capes.gov.br/webqualis/publico/pesquisaPublicaClassificacao.seam?conversationPropagation=begin) and it was computed the number of guidance of students scientific projects. RESULTS: Seventy-four professors were included, 31 with Master and 43 with PhD degrees. UFPA presented more publications (49% out of the total), which 32% of them in Journals B1 or higher. Otorhinolaryngology presented the greatest number of publications (29% out of the total); however, 98% of them in B3 or lower. Gastroenterological Surgery, which is responsible for 23% out of the total, had 57% of its publications in B1 or higher. The most frequent type of guidance was the one about scientific work of completion of undergraduate course (63%). Gynecology and Obstetrics presented the greatest number of student's guidance (35% out of the total) and Gastroenterological Surgery the largest number of Master's degree and PhD degree supervisions. CONCLUSIONS: The specialties Gynecology and Obstetrics and Gastroenterological Surgery from the State University of Para (UEPA), Federal Universities of Para (UFPA) and of Paraiba (UFPB) presented the greatest number of professors with academic degrees. Gastroenterological Surgery presented the largest scientific productions at higher strata of WebQualis CAPES classification and also the largest number of Master and PhD directed.

Perfil dos cuidadores e as dificuldades enfrentadas no cuidado ao idoso, em Ananindeua, PA / Profile of caregivers and difficulties in elderly care in the city of Ananindeua, State of Para, Brazil

OBJETIVO: Descrever o perfil dos cuidadores de idoso, bem como sua importância e principais dificuldades no ato de cuidar do idoso. METODOLOGIA: Trata-se de estudo transversal descritivo e exploratório realizado pela equipe PET Saúde, em uma Estratégia de Saúde da Família (ESF), no município de Ananindeua, Pará. A coleta de dados foi feita por meio de um questionário semiestruturado abordando aspectos sociodemográficos e questões referentes a importância e dificuldades encontradas pelos cuidadores de idosos. RESULTADOS: Foram entrevistados 31 cuidadores, predominando sexo feminino, estado civil casado, com ensino médio completo, renda entre dois e três salários mínimos, tempo de exercício da função maior que 12 meses, em sua abrangência cuidadores informais e com vínculo de parentesco com o idoso. Os cuidadores reconheceram que é importante a presença de um cuidador e consideraram o idoso sob seu cuidado como saudável. CONCLUSÃO: A maioria dos cuidadores era do tipo informal, caracterizada por mulheres, com vínculo familiar, que destacaram a paciência e a falta de conhecimento como as principais dificuldades do cuidador. Os dados deste estudo se limitam a uma realidade local, necessitando de mais pesquisas para fomentar as particularidades e dificuldades enfrentadas pelos cuidadores.

OBJECTIVE: To describe the profile of caregivers of elderly, as well as their importance and main difficulties in caring for the elderly. METHODOLOGY: This was a descriptive, exploratory cross-sectional study conducted by the PET Saúde staff in a Family Health Strategy (FHS) in the city of Ananindeua, state of Pará, Brazil. Data was collected through a semistructured questionnaire addressing sociodemographic and issues concerning the importance and difficulties encountered by caregivers of the elderly. RESULTS: We interviewed 31 caregivers, predominantly female, married state, with complete secondary education, income between two and three minimum wages, time of exercise of the function over 12 months, most of them informal caregivers and with family relationship with elderly. Caregivers acknowledged the importance of a caregiver and found the elderly under their care as healthy. CONCLUSIONS: Most caregivers were informal type, characterized by women with family ties, which emphasized patience and lack of knowledge as the main difficulties of the caregiver. Data from this study are limited to a local reality, requiring further research to foster the particularities and difficulties faced by caregivers.